18612924577,18511767098
詳細介紹
公司介紹
北京(jing)北納創聯生物技術研(yan)究院是(shi)一家專業從事(shi)標(biao)準(zhun)物質(zhi)、計量分(fen)析儀器和精細化(hua)工(gong)產品研(yan)發及(ji)銷(xiao)售的科研(yan)單位。是(shi)國內標(biao)準(zhun)物質(zhi)提(ti)供(gong)商之一，代理銷(xiao)售國內外數萬種標(biao)準(zhun)物質(zhi)、微生物、菌種、細胞(bao)類等產品。
北(bei)京標(biao)準物(wu)質網隸屬于北(bei)京北(bei)納(na)創聯生物(wu)技術(shu)研究院(yuan)(yuan)，具備強大的分析測(ce)試、技術(shu)開發(fa)、*、物(wu)流(liu)管理和售后服務能(neng)力，并與國內(nei)外眾多(duo)檢測(ce)機構、科(ke)研院(yuan)(yuan)校和工廠企業有(you)著良好和穩定的合(he)作關系，具有(you)較(jiao)強的性(xing)和影(ying)響(xiang)力。
北納生(sheng)(sheng)物也隸屬于北京北納創聯(lian)生(sheng)(sheng)物技術(shu)研究(jiu)院，實(shi)(shi)驗(yan)基(ji)地總(zong)占地面積16畝(mu)，并設(she)有(you)細(xi)胞(bao)庫(ku)、微生(sheng)(sheng)物菌種保(bao)藏(zang)庫(ku)，細(xi)胞(bao)培(pei)養(yang)、菌種培(pei)養(yang)等實(shi)(shi)驗(yan)室。擁有(you)細(xi)胞(bao)和菌株(zhu)2萬余株(zhu)，并設(she)有(you)生(sheng)(sheng)物資源信息(xi)庫(ku)，收錄信息(xi)達(da)20余萬株(zhu)。致力于提供實(shi)(shi)驗(yan)室綜合(he)解決方案，為廣(guang)大客戶服務。
經營范圍：
國(guo)家標(biao)(biao)準(zhun)物質、國(guo)內外標(biao)(biao)準(zhun)品(pin)、對照品(pin)、實驗耗(hao)材(cai)、試劑(ji)、菌株(zhu)、細(xi)胞、ATCC、培養基、血清、冶金(jin)標(biao)(biao)樣、標(biao)(biao)準(zhun)氣體、試劑(ji)盒等(deng)，歡迎查(cha)詢！
ATCCCCL-139 TCMK-1 ATCC細胞購買
| 基本信息 | |
| 資源編號 | ATCCCCL-139 |
| 資源名稱 | TCMK-1 |
| 種屬 | Mus musculus, mouse kidney |
| 類型 | SV40 transformed |
| 形態 | epithelial |
| 提供形式 | frozen |
| 致病類型 | normal |
| 安全等級 | 2 |
| 培養方法 | |
| 培養基 | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| 傳代方法 | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| 生長條件 | Temperature: 37°C |
| 生長特性 | adherent |
| 存儲條件 | liquid nitrogen vapor phase;Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| 詳細說明 | |
| 描述 | About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable. Tested and found negative for ectromelia virus (mousepox). |
| Age | weanling |
| Strain | C3H/Mai |
| Karyotype | modal number = 78; range = 64 to 157.All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes. |
| Derivation | The cells are SV40 transformed and are positive for SV40 T-antigen. |
| Antigen Expression | H-2k |
| Tumorigenic | No |
| Effects | No, in immunosuppressed mice Yes, in semisolid medium |
| Virus Susceptibility | Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1 |
| Virus Resistance | poliovirus 1 |
| Year of Origin | 1963 |
ATCCCCL-139 TCMK-1 ATCC細胞購買
溫(wen)馨提示：
本店(dian)商品種類眾(zhong)多，因鋪位有(you)限商品未全部上架，如果(guo)沒有(you)找到您想(xiang)要的(de)商品可以咨詢(xun)客服(fu)，我們會耐心解答，祝您購(gou)物愉快！
產品咨詢
技術支持： sitemap.xml
歡迎來到
